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• Journal article
  Mercedes Palomino M, Allievi MC, Gruendling A, Sanchez-Rivas C, Ruzal SMet al., 2013,
  , MICROBIOLOGY-SGM, Vol: 159, Pages: 2416-2426, ISSN: 1350-0872
  • Cite
  • Citations: 41
• Journal article
  Quigley A, Heng JYY, Liddell JM, Williams DRet al., 2013,
  , EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, Vol: 85, Pages: 1103-1111, ISSN: 0939-6411
  • Cite
  • Citations: 12
• Journal article
  Bebeacua C, Tremblay D, Farenc C, Chapot-Chartier M-P, Sadovskaya I, van Heel M, Veesler D, Moineau S, Cambillau Cet al., 2013,
  , JOURNAL OF VIROLOGY, Vol: 87, Pages: 12302-12312, ISSN: 0022-538X
  • Cite
  • Citations: 82
• Journal article
  Bertheleme N, Chae PS, Singh S, Mossakowska D, Hann MM, Smith KJ, Hubbard JA, Dowell SJ, Byrne Bet al., 2013,
  , BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, Vol: 1828, Pages: 2583-2591, ISSN: 0005-2736
  • Cite
  • Citations: 31
• Journal article
  Ali SSM, Heng JYY, Nikolaev AA, Waters KEet al., 2013,
  , POWDER TECHNOLOGY, Vol: 249, Pages: 373-377, ISSN: 0032-5910
  • Cite
  • Citations: 45
• Journal article
  Zhou Z, Mogensen MM, Powell PP, Curry S, Wileman Tet al., 2013,
  , JOURNAL OF VIROLOGY, Vol: 87, Pages: 11721-11729, ISSN: 0022-538X
  • Cite
  • Citations: 35
• Journal article
  Bialek W, Wen S, Michoux F, Beckova M, Komenda J, Murray JW, Nixon PJet al., 2013,
  , PHOTOSYNTHESIS RESEARCH, Vol: 117, Pages: 375-383, ISSN: 0166-8595
  • Cite
  • Citations: 10
• Journal article
  Casini A, MacDonald JT, De Jonghe J, Christodoulou G, Freemont PS, Baldwin GS, Ellis Tet al., 2013,
  , Nucleic Acids 91��ɫ, Vol: 42, ISSN: 1362-4962

  Overlap-directed DNA assembly methods allowmultiple DNA parts to be assembled together inone reaction. These methods, which rely onsequence homology between the ends of DNAparts, have become widely adopted in syntheticbiology, despite being incompatible with a key principleof engineering: modularity. To answer this, wepresent MODAL: a Modular Overlap-DirectedAssembly with Linkers strategy that brings modularityto overlap-directed methods, allowing assemblyof an initial set of DNA parts into a variety ofarrangements in one-pot reactions. MODAL isaccompanied by a custom software tool thatdesigns overlap linkers to guide assembly,allowing parts to be assembled in any specifiedorder and orientation. The in silico design of syntheticorthogonal overlapping junctions allows formuch greater efficiency in DNA assembly for avariety of different methods compared with usingnon-designed sequence. In tests with three differentassembly technologies, the MODAL strategy givesassembly of both yeast and bacterial plasmids,composed of up to five DNA parts in the kilobaserange with efficiencies of between 75 and 100%.It also seamlessly allows mutagenesis to beperformed on any specified DNA parts duringthe process, allowing the one-step creation of constructlibraries valuable for synthetic biologyapplications.

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• Journal article
  Sethurathinam S, Singh LP, Panneerselvam P, Byrne B, Ding JLet al., 2013,
  , FEBS LETTERS, Vol: 587, Pages: 3296-3302, ISSN: 0014-5793
  • Cite
  • Citations: 13
• Journal article
  Gruendling A, 2013,
  , MBIO, Vol: 4, ISSN: 2150-7511
  • Cite
  • Citations: 42
• Journal article
  Solomon HV, Tabachnikov O, Feinberg H, Govada L, Chayen NE, Shoham Y, Shoham Get al., 2013,
  , ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Vol: 69, Pages: 1114-1119
  • Cite
  • Citations: 22
• Journal article
  Carafoli F, Hohenester E, 2013,
  , BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Vol: 1834, Pages: 2187-2194, ISSN: 1570-9639
  • Cite
  • Citations: 88
• Journal article
  Canning P, Cooper CDO, Krojer T, Murray JW, Pike ACW, Chaikuad A, Keates T, Thangaratnarajah C, Hojzan V, Ayinampudi V, Marsden BD, Gileadi O, Knapp S, von Delft F, Bullock ANet al., 2013,
  , JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 288, Pages: 28304-28304
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• Journal article
  De Simone A, Gustavsson M, Montalvao RW, Shi L, Veglia G, Vendruscoo Met al., 2013,
  , BIOCHEMISTRY, Vol: 52, Pages: 6684-6694, ISSN: 0006-2960
  • Cite
  • Citations: 22
• Journal article
  Yip GM, Chen ZW, Edge CJ, Smith EH, Dickinson R, Hohenester E, Townsend RR, Fuchs K, Sieghart W, Evers AS, Franks NPet al., 2013,
  , Nature Chemical Biology, Vol: 9, Pages: 715-720, ISSN: 1552-4469

  Propofol is the most important intravenous general anesthetic in current clinical use. It acts by potentiating GABAA (γ-aminobutyric acid type A) receptors, but where it binds to this receptor is not known and has been a matter of some debate. We synthesized a new propofol analog photolabeling reagent whose biological activity is very similar to that of propofol. We confirmed that this reagent labeled known propofol binding sites in human serum albumin that have been identified using X-ray crystallography. Using a combination of protiated and deuterated versions of the reagent to label mammalian receptors in intact membranes, we identified a new binding site for propofol in GABAA receptors consisting of both β3 homopentamers and α1β3 heteropentamers. The binding site is located within the β subunit at the interface between the transmembrane domains and the extracellular domain and lies close to known determinants of anesthetic sensitivity in the transmembrane segments TM1 and TM2.

  • Abstract
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• Journal article
  Pearson JS, Giogha C, Ong SY, Kennedy CL, Kelly M, Robinson KS, Lung TWF, Mansell A, Riedmaier P, Oates CVL, Zaid A, Muehlen S, Crepin VF, Marches O, Ang C-S, Williamson NA, O'Reilly LA, Bankovacki A, Nachbur U, Infusini G, Webb AI, Silke J, Strasser A, Frankel G, Hartland ELet al., 2013,
  , NATURE, Vol: 501, Pages: 247-+, ISSN: 0028-0836
  • Cite
  • Citations: 228
• Journal article
  Choudhury HG, Beis K, 2013,
  , PROTEIN SCIENCE, Vol: 22, Pages: 1287-1293, ISSN: 0961-8368
  • Cite
  • Citations: 13
• Journal article
  Reichmann NT, Cassona CP, Gruendling A, 2013,
  , MICROBIOLOGY-SGM, Vol: 159, Pages: 1868-1877, ISSN: 1350-0872
  • Cite
  • Citations: 77
• Journal article
  Saridakis E, Chayen NE, 2013,
  , TRENDS IN BIOTECHNOLOGY, Vol: 31, Pages: 515-520, ISSN: 0167-7799
  • Cite
  • Citations: 45
• Journal article
  Filloux A, 2013,
  , NATURE, Vol: 500, Pages: 284-285, ISSN: 0028-0836
  • Cite
  • Citations: 8
• Journal article
  Moore BL, Kelley LA, Barber J, Murray JW, MacDonald JTet al., 2013,
  , JOURNAL OF COMPUTATIONAL CHEMISTRY, Vol: 34, Pages: 1881-1889, ISSN: 0192-8651
  • Cite
  • Citations: 50
• Journal article
  Squire JM, Guerreiro MJ, Sidebotham RL, Reis CA, Wiseman J, Luther PKet al., 2013,
  , ANALYTICAL BIOCHEMISTRY, Vol: 439, Pages: 204-211, ISSN: 0003-2697
  • Cite
  • Citations: 3
• Journal article
  Krupnik T, Kotabova E, van Bezouwen LS, Mazur R, Garstka M, Nixon PJ, Barber J, Kana R, Boekema EJ, Kargul Jet al., 2013,
  , JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 288, Pages: 23529-23542, ISSN: 0021-9258
  • Cite
  • Citations: 52
• Journal article
  Mikkelsen H, Hui K, Barraud N, Filloux Aet al., 2013,
  , Molecular Microbiology, Vol: 89, Pages: 450-463, ISSN: 0950-382X

  Pseudomonas aeruginosa biofilm formation is linked to persistent infections in humans. Biofilm formation is facilitated by extracellular appendages, some of which are assembled by the Chaperone Usher Pathway (Cup). The cupD gene cluster is located on the PAPI‐1 pathogenicity island of strain PA14 and has probably been acquired together with four genes encoding two‐component signal transduction proteins. We have previously showed that the RcsB response regulator activates expression of the cupD genes, which leads to the production of CupD fimbriae and increased attachment. Here we show that RcsB activity is tightly modulated by two sensors, RcsC and PvrS. While PvrS acts as a kinase that enhances RcsB activity, RcsC has a dual function, first as a phosphorelay, and second as a phosphatase. We found that, under certain growth conditions, overexpression of RcsB readily induces biofilm dispersal. Microarray analysis shows that RcsB positively controls expression of pvrR that encodes the phosphodiesterase required for this dispersal process. Finally, in addition to the PAPI‐1 encoded cupD genes, RcsB controls several genes on the core genome, some of which encode orphan response regulators. We thus discovered that RcsB is central to a large regulatory network that fine‐tunes the switch between biofilm formation and dispersal.

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• Journal article
  Raymond B, Young JC, Pallett M, Endres RG, Clements A, Frankel Get al., 2013,
  , TRENDS IN MICROBIOLOGY, Vol: 21, Pages: 430-439, ISSN: 0966-842X
  • Cite
  • Citations: 109
• Journal article
  Corrigan RM, Gruendling A, 2013,
  , NATURE REVIEWS MICROBIOLOGY, Vol: 11, Pages: 513-524, ISSN: 1740-1526
  • Cite
  • Citations: 298
• Journal article
  Carafoli F, Hamaia SW, Bihan D, Hohenester E, Farndale RWet al., 2013,
  , PLOS ONE, Vol: 8, ISSN: 1932-6203
  • Cite
  • Citations: 31
• Journal article
  Kato M, Cardona T, Rutherford AW, Reisner Eet al., 2013,
  , JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 135, Pages: 10610-10613, ISSN: 0002-7863
  • Cite
  • Citations: 106
• Journal article
  Zhu M, De Simone A, Schenk D, Toth G, Dobson CM, Vendruscolo Met al., 2013,
  , JOURNAL OF CHEMICAL PHYSICS, Vol: 139, ISSN: 0021-9606
  • Cite
  • Citations: 71
• Journal article
  Santos AJM, Meinecke M, Fessler MB, Holden DW, Boucrot Eet al., 2013,
  , JOURNAL OF CELL SCIENCE, Vol: 126, Pages: 2990-2996, ISSN: 0021-9533
  • Cite
  • Citations: 32

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